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nrg1 b1 hrg b1  (R&D Systems)


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    R&D Systems nrg1 b1 hrg b1
    Nrg1 B1 Hrg B1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+nrg1+beta/Recombinant+Human+NRG1-beta+1%2FHRG1-beta+1+EGF+Domain+Protein/10__1158_slash_0008___5472__can___25___4018-282-40-42
    Average 95 stars, based on 124 article reviews
    nrg1 b1 hrg b1 - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: Unbiased Combinatorial Screening Identifies a Bispecific IgG1 that Potently Inhibits HER3 Signaling via HER2-Guided Ligand Blockade.
    Article Snippet: Recombinant Fc-tagged human EGFR, HER2, HER3 and HER4 extracellular domain (ECD) proteins were purchased from R&D Systems (Cat# 344-ER, 1129-ER, 348-RB, 1131-ER). .. Recombinant human NRG1-beta 1/HRG1-beta 1 EGF-like domain protein was purchased from R&D Systems (Cat#396-HB) and Prospec Bio (Cat#Cyt-733). .. Recombinant human EGF was purchased from DiscoverX (Cat#92-1113).

    Article Title: Inhibitory Neural Network’s Impairments at Hippocampal CA1 LTP in an Aged Transgenic Mouse Model of Alzheimer’s Disease
    Article Snippet: .. Recombinant human NRG1-beta 1/HRG1-beta 1 EGF domain proteins (396-HB-050; R&D Systems, Minneapolis, MN, USA), reconstituted in sterile PBS containing at least 0.1% human or bovine serum albumin to a final concentration of at 100 μg/mL. .. PD158780 (513035; Calbiochem, Darmstadt, Germany) and reconstituted in dimethyl sulfoxide (5 mg/mL).

    Article Title: C5aR plus MEK inhibition durably targets the tumor milieu and reveals tumor cell phagocytosis
    Article Snippet: .. Neurons and fibroblasts were removed from cultures, cells maintained for 7 d, then trypsinized and replated in a six well dish and expanded until confluent in Schwann cell growth medium containing 2 μM forskolin (Cayman Chemical Company), 10 ng/ml recombinant human NRG1-beta 1/HRG1-beta 1 EGF Domain peptide (Cat# 396-HB-050; R&D Systems), and 10% FBS. .. Schwann cells were labeled with 5 μm of CFSE (BioLegend) at 37°C for 20 min, thoroughly washed with PBS, and incubated in warmed Opti-MEM for 30 min. BMM were treated with PD0325901 (1 μM), A8Δ 71–73 (1 μM), or vehicle control for 8 h. Schwann cells were trypsinized, washed, and 8 × 10 4 labeled Schwann were added to pre-treated BMM.

    Article Title: Claudin-15 overexpression inhibits proliferation and promotes apoptosis of Schwann cells in vitro
    Article Snippet: Fibroblasts were destroyed by complement cleavage using polyclonal anti-Thy1.1 antiserum (Sigma). .. Recombinant human NRG1-beta 1/HRG1-beta 1 epidermal growth factor Domain Protein (R&D system, Minneapolis, MN, USA) and forskolin (Sigma) were added to the media for cell proliferation. .. Schwann cells were transfected separately with siRNA-1, siRNA-2 and siRNA-3 (RiboBio, Guangzhou, China; ) using Lipofectamine 3000 reagent (Invitrogen, New York, NJ, USA) for Claudin-15 knockdown ( n = 3).

    Article Title: Neuregulin antagonists and use thereof in treating cancer
    Article Snippet: .. Addition of exogenous NRG1 on H441 cells were performed as follows: H441 cells were serum starved for 18 hours prior to addition of 1 uM recombinant human NRG1 beta-1 extracellular domain (R&D systems) or 1 uM anti-ragweed IgG2A as the control. ..

    Article Title: Chemokine platelet factor 4 accelerates peripheral nerve regeneration by regulating Schwann cell activation and axon elongation.
    Article Snippet: Isolated cells were cultured in Dulbecco’s modified Eagle medium (Corning, Corning, NY, USA) containing 10% fetal bovine serum (Gibco) and 1% penicillinstreptomycin (ScienCell, Carlsbad, CA, USA) and incubated with anti-Thy1.1 antibody (Sigma) and rabbit complement (Invitrogen, Carlsbad, CA, USA) to remove contaminating fibroblasts. .. Purified Schwann cells were treated with 2 μM forskolin (Sigma) and 10 ng/mL recombinant human NRG1-beta 1/HRG1beta 1 EGF domain (HRG; R&D Systems Inc., Minneapolis, MN, USA) until they reached confluence. .. Schwann cells cultured in fetal bovine serum-containing medium were transfected with small interfering RNA (siRNA) against PF4 (target sequence: 5′-GAA AGC GAC GGA GAT CTT A-3′; Ribobio, Guangzhou, Guangdong, China) or control siRNA (target sequence: 5′-UUC UCC GAA CGU GUC ACG UTT-3′; Ribobio) using LipofectamineTM RNAiMAX (Invitrogen) for at least 24 hours to examine the effect of PF4 knockdown.

    Article Title: Chemokine platelet factor 4 accelerates peripheral nerve regeneration by regulating Schwann cell activation and axon elongation
    Article Snippet: Isolated cells were cultured in Dulbecco’s modified Eagle medium (Corning, Corning, NY, USA) containing 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (ScienCell, Carlsbad, CA, USA) and incubated with anti-Thy1.1 antibody (Sigma) and rabbit complement (Invitrogen, Carlsbad, CA, USA) to remove contaminating fibroblasts. .. Purified Schwann cells were treated with 2 μM forskolin (Sigma) and 10 ng/mL recombinant human NRG1-beta 1/HRG1-beta 1 EGF domain (HRG; R&D Systems Inc., Minneapolis, MN, USA) until they reached confluence. .. Schwann cells cultured in fetal bovine serum-containing medium were transfected with small interfering RNA (siRNA) against PF4 (target sequence: 5′-GAA AGC GAC GGA GAT CTT A-3′; Ribobio, Guangzhou, Guangdong, China) or control siRNA (target sequence: 5′-UUC UCC GAA CGU GUC ACG UTT-3′; Ribobio) using LipofectamineTM RNAiMAX (Invitrogen) for at least 24 hours to examine the effect of PF4 knockdown.

    Article Title: erbB3 Is an Active Tyrosine Kinase Capable of Homo- and Heterointeractions
    Article Snippet: 90 HRG-β1 was from US Biological (Swampscott, MA), staurosporine from Calbiochem (La Jolla, CA) 91 and lapatinib from Eton Biosciences Inc. (San Diego, CA). .. Recombinant human NRG1-beta 1/HRG1-92 beta 1 extracellular domain (R&D Systems, Minneapolis, MN) was singly biotinylated using NHS-ester 93 conjugation chemistry (Biotin-XX, sulfosuccinimidyl ester, Invitrogen). .. HRG-biotin and anti-HA Fab-94 biotin (Roche) were conjugated to Qdot® 655 or Qdot® 585 streptavidin conjugate (Invitrogen) in PBS 95 + 1% BSA to generate stock solutions of 30 nM 1:1 monovalent QD-conjugates.

    Sterility:

    Article Title: Inhibitory Neural Network’s Impairments at Hippocampal CA1 LTP in an Aged Transgenic Mouse Model of Alzheimer’s Disease
    Article Snippet: .. Recombinant human NRG1-beta 1/HRG1-beta 1 EGF domain proteins (396-HB-050; R&D Systems, Minneapolis, MN, USA), reconstituted in sterile PBS containing at least 0.1% human or bovine serum albumin to a final concentration of at 100 μg/mL. .. PD158780 (513035; Calbiochem, Darmstadt, Germany) and reconstituted in dimethyl sulfoxide (5 mg/mL).

    Concentration Assay:

    Article Title: Inhibitory Neural Network’s Impairments at Hippocampal CA1 LTP in an Aged Transgenic Mouse Model of Alzheimer’s Disease
    Article Snippet: .. Recombinant human NRG1-beta 1/HRG1-beta 1 EGF domain proteins (396-HB-050; R&D Systems, Minneapolis, MN, USA), reconstituted in sterile PBS containing at least 0.1% human or bovine serum albumin to a final concentration of at 100 μg/mL. .. PD158780 (513035; Calbiochem, Darmstadt, Germany) and reconstituted in dimethyl sulfoxide (5 mg/mL).

    Control:

    Article Title: Neuregulin antagonists and use thereof in treating cancer
    Article Snippet: .. Addition of exogenous NRG1 on H441 cells were performed as follows: H441 cells were serum starved for 18 hours prior to addition of 1 uM recombinant human NRG1 beta-1 extracellular domain (R&D systems) or 1 uM anti-ragweed IgG2A as the control. ..

    Purification:

    Article Title: Chemokine platelet factor 4 accelerates peripheral nerve regeneration by regulating Schwann cell activation and axon elongation.
    Article Snippet: Isolated cells were cultured in Dulbecco’s modified Eagle medium (Corning, Corning, NY, USA) containing 10% fetal bovine serum (Gibco) and 1% penicillinstreptomycin (ScienCell, Carlsbad, CA, USA) and incubated with anti-Thy1.1 antibody (Sigma) and rabbit complement (Invitrogen, Carlsbad, CA, USA) to remove contaminating fibroblasts. .. Purified Schwann cells were treated with 2 μM forskolin (Sigma) and 10 ng/mL recombinant human NRG1-beta 1/HRG1beta 1 EGF domain (HRG; R&D Systems Inc., Minneapolis, MN, USA) until they reached confluence. .. Schwann cells cultured in fetal bovine serum-containing medium were transfected with small interfering RNA (siRNA) against PF4 (target sequence: 5′-GAA AGC GAC GGA GAT CTT A-3′; Ribobio, Guangzhou, Guangdong, China) or control siRNA (target sequence: 5′-UUC UCC GAA CGU GUC ACG UTT-3′; Ribobio) using LipofectamineTM RNAiMAX (Invitrogen) for at least 24 hours to examine the effect of PF4 knockdown.

    Article Title: Chemokine platelet factor 4 accelerates peripheral nerve regeneration by regulating Schwann cell activation and axon elongation
    Article Snippet: Isolated cells were cultured in Dulbecco’s modified Eagle medium (Corning, Corning, NY, USA) containing 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (ScienCell, Carlsbad, CA, USA) and incubated with anti-Thy1.1 antibody (Sigma) and rabbit complement (Invitrogen, Carlsbad, CA, USA) to remove contaminating fibroblasts. .. Purified Schwann cells were treated with 2 μM forskolin (Sigma) and 10 ng/mL recombinant human NRG1-beta 1/HRG1-beta 1 EGF domain (HRG; R&D Systems Inc., Minneapolis, MN, USA) until they reached confluence. .. Schwann cells cultured in fetal bovine serum-containing medium were transfected with small interfering RNA (siRNA) against PF4 (target sequence: 5′-GAA AGC GAC GGA GAT CTT A-3′; Ribobio, Guangzhou, Guangdong, China) or control siRNA (target sequence: 5′-UUC UCC GAA CGU GUC ACG UTT-3′; Ribobio) using LipofectamineTM RNAiMAX (Invitrogen) for at least 24 hours to examine the effect of PF4 knockdown.

    Conjugation Assay:

    Article Title: erbB3 Is an Active Tyrosine Kinase Capable of Homo- and Heterointeractions
    Article Snippet: 90 HRG-β1 was from US Biological (Swampscott, MA), staurosporine from Calbiochem (La Jolla, CA) 91 and lapatinib from Eton Biosciences Inc. (San Diego, CA). .. Recombinant human NRG1-beta 1/HRG1-92 beta 1 extracellular domain (R&D Systems, Minneapolis, MN) was singly biotinylated using NHS-ester 93 conjugation chemistry (Biotin-XX, sulfosuccinimidyl ester, Invitrogen). .. HRG-biotin and anti-HA Fab-94 biotin (Roche) were conjugated to Qdot® 655 or Qdot® 585 streptavidin conjugate (Invitrogen) in PBS 95 + 1% BSA to generate stock solutions of 30 nM 1:1 monovalent QD-conjugates.



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